| Autor: |
Campanacci, Valérie, Egloff, Marie-Pierre, Longhi, Sonia, Ferron, França;ois, Rancurel, Corinne, Salomoni, Aurelia, Durousseau, Cécile, Tocque, Fabienne, Brémond, Nicolas, Dobbe, Jessika C., Snijder, Eric J., Canard, Bruno, Cambillau, Christian |
| Předmět: |
|
| Zdroj: |
Acta Crystallographica: Section D (Wiley-Blackwell); Sep2003, Vol. 59 Issue 9, p1628, 4p |
| Abstrakt: |
The actiologic agent of the recent epidemics of Severe Acute Respiratory Syndrome (SARS) is a positive-stranded RNA virus (SARS-CoV) belonging to the Coronaviridae family and its genome differs substantially from those of other known coronaviruses. SARS-CoV is transmissible mainly by the respiratory route and to date there is no vaccine and no prophylactic or therapeutic treatments against this agent. A SARS-CoV whole-genome approach has been developed or domains. These studies are expected to greatly facilitate drug design. The genomes of coronaviruses are between 27 and 31.5 kbp in length, the largest of the known RNA viruses, and encode 20-30 mature proteins. The functions of many of these polypeptides, including the Nsp9-Nsp-10 replicase-cleavage products, are still unknown. Here, the cloning, Escherichia coli expression, purification and crystallization of the SARS-CoV Nsp9 protein, the first SARS-CoV protein to be crystallized, are reported Nsp9 crystals diffract to 2.8 Å resolution and belong to space group P6⅕ with unit-cell parameters a = b = 89.7, c = 136.7 Å. With two molecules in the asymmetric unit, the solvent content is 60% (V[SUBM] = 3.1 Å[SUP3]Da-1). [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
