Autor: |
Cao, Xiaopei, Gao, Zhiyong, Robert, Claudia E., Greene, Scott, Xu, Gang, Xu, Weizhen, Bell, Ewan, Campbell, Don, Zhu, Yuan, Young, Robert, Trucco, Matteo, Markmann, James F., Naji, Ali, Wolf, Bryan A. |
Předmět: |
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Zdroj: |
Diabetes; Sep2003, Vol. 52 Issue 9, p2296-2303, 8p, 2 Color Photographs, 2 Black and White Photographs, 5 Graphs |
Abstrakt: |
PANDER (PANcreatic DERived factor, FAM3B), a newly discovered secreted cytokine, is specifically expressed at high levels in the islets of Langerhans of the endocrine pancreas. To evaluate the role of PANDER in β-cell function, we investigated the effects of PANDER on rat, mouse, and human pancreatic islets; the β-TC3 cell line; and the α-TC cell line. PANDER protein was present in α- and β-cells of pancreatic islets, insulinsecreting β-TC3 cells, and glucagon-secreting α-TC cells. PANDER induced islet cell death in rat and human islets. Culture of β-TC3 cells with recombinant PANDER had a dose-dependent inhibitory effect on cell viability. This effect was also time-dependent. PANDER caused apoptosis of β-cells as assessed by electron microscopy, annexin V fluorescent staining, and flow-cytometric terminal deoxynucleotidyl transferase-mediated dUTP nickend labeling assay. PANDER did not affect cytosollc Ca[sup 2+] levels or nitric oxide levels. However, PANDER activated caspase-3. Hence, PANDER may have a role in the process of pancreatic β-cell apoptosis. [ABSTRACT FROM AUTHOR] |
Databáze: |
Complementary Index |
Externí odkaz: |
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