| Abstrakt: |
Purpose: To understand the contribution of stromal cells, such as granulation tissue fibroblasts, to peri-implantitis with regard to (1) the secretion of constitutive factors promoting migration/survival of infiltrates into osseointegrated sites; and (2) the effect of exogenous infiltrate cytokines on the cells' secretion. Materials and Methods: Fibroblasts were cultured from eight peri-implantitis sites. Multiplexed enzyme-linked immunosorbent assay was used to quantify factors secreted by the cells either unstimulated or stimulated with gamma interferon (lFNy), interleukin 4 (lL4), or tumor necrosis factor alpha (TNFa). Controls consisted of fibroblasts cultured from healthy gingival and chronic periodontitis granulation tissues. Results: Peri-implantitis fibroblasts differed significantly from periodontitis fibro blasts in their reduced secretion of the collagen inducer transforming growth factor beta-l (TGFf31) and tissue inhibitor of metalloproteinase-1. The cells exhibited enhanced secretion of angiogenic factor vascular endothelial growth factor (VEGF) and collagenolytic matrix metalloproteinase 1 (MMP1) compared to both healthy and periodontitis fibroblasts. Fibroblasts from both periodontitis and periimplantitis sites exhibited a pronounced proinflammatory profile compared to normal gingival fibro blasts with respect to secretion of chemokines IL6, ILB, and monocyte chemoattractant protein 1 (MCP1). Fibroblasts stimulated with TNFa showed increased levels of IL6, ILB, MCP1; neutrophil chemokine growth-related oncogene alpha stimulation with IFNy increased MCP1; and stimulation with IL4 increased VEGF. Conclusion: The results indicate that peri-implantitis fibroblasts represent a distinct stromal population. The cells might participate in the pathogenesis of peri-implantitis by up regulating both vascularity and matrix breakdown, thus promoting migration/maintenance of infiltrates into the site. Cytokines produced by infiltrates could enhance the inflammatory nature of the cells in a self-feeding loop. INT J ORAL MAXILLOFAC IMPLANTS 2009;24:197-204 [ABSTRACT FROM AUTHOR] |
