| Autor: |
Helbig L, Yaromina A, Sriramareddy SN, Böke S, Koi L, Thames HD, Baumann M, Zips D, Helbig, L, Yaromina, A, Sriramareddy, S N, Böke, S, Koi, L, Thames, H D, Baumann, M, Zips, D |
| Zdroj: |
Strahlentherapie und Onkologie; Nov2012, Vol. 188 Issue 11, p1031-1037, 7p |
| Abstrakt: |
Background and Purpose: Hypoxia and reoxygenation are important determinants of outcome after radiotherapy. HIF-1α is a key molecule involved in cellular response to hypoxia. HIF-1α expression levels have been shown to change after irradiation. The objective of the present study was to explore the prognostic value of HIF-1α expression during fractionated irradiation.Materials and Methods: Six human squamous cell carcinoma models xenografted in nude mice were analysed. Tumours were excised after 3, 5 and 10 fractions. HIF-1α expression was quantified by western blot. For comparative analysis, previously published data on local tumour control data and pimonidazole hypoxic fraction was used.Results: HIF-1α expression in untreated tumours exhibited intertumoural heterogeneity and did not correlate with pimonidazole hypoxic fraction. During fractionated irradiation the majority of tumour models exhibited a decrease in HIF-1α expression, whereas in UT-SCC-5 no change was observed. Neither kinetics nor expression levels during fractionated irradiation correlated with local tumour control.Conclusion: Our data do not support the use of HIF-1α determined during treatment as a biomarker to predict outcome after fractionated irradiation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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