NOTCH and NF-κB interplay in chronic lymphocytic leukemia is independent of genetic lesion.

Autor: Baldoni, Stefano, Sportoletti, Paolo, Del Papa, Beatrice, Aureli, Patrizia, Dorillo, Erica, Rosati, Emanuela, Ciurnelli, Raffaella, Marconi, Pierfrancesco, Falzetti, Franca, Di Ianni, Mauro
Zdroj: International Journal of Hematology; Aug2013, Vol. 98 Issue 2, p153-157, 5p
Abstrakt: The NOTCH and nuclear factor kappa B (NF-κB) pathways are both constitutively activated in Chronic Lymphocytic Leukemia (CLL). We first described the NOTCH1 PEST domain mutation in a CLL subgroup, but the activation of the NOTCH pathway in NOTCH1-unmutated cases remains unexplained. Here, we investigated whether genetic lesions in the NF-κB/NOTCH loop might support the NOTCH activation status by sequencing negative (TNFAIP3/A20) and positive (TRAF2, TRAF5, TNFRSF11A/RANK, MAP3K7/TAK1, and CARD11) regulators of NF-κB together with NF-κB targets on the NOTCH pathway, the NOTCH ligands Jagged1 and Jagged2, in CLL patients. The sequence analysis revealed four missense mutations for A20, TRAF2, TRAF5 and RANK1 genes, all causing a change in amino acid group from polar to non-polar, but functional domains were not involved. Specific predictive software analyses confirmed that the amino acid changes have a low-functional impact on the protein. Our results show that in CLL, NF-κB regulators and Jagged are both unmutated, suggesting that the Jagged-mediated interplay between NF-κB and NOTCH is independent of genetic lesions. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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