| Autor: |
Bogen, Oliver, Bender, Olaf, Löwe, Jana, Blenau, Wolfgang, Thevis, Beatrice, Schröder, Wolfgang, Margolis, Richard U., Levine, Jon D., Hucho, Ferdinand |
| Předmět: |
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| Zdroj: |
Journal of Neurochemistry; Jul2015, Vol. 134 Issue 1, p147-155, 9p |
| Abstrakt: |
A subpopulation of nociceptors, the glial cell line-derived neurotrophic factor ( GDNF)-dependent, non-peptidergic C-fibers, expresses a cell-surface glycoconjugate that can be selectively labeled with isolectin B4 ( IB4), a homotetrameric plant lectin from Griffonia simplicifolia. We show that versican is an IB4-binding molecule in rat dorsal root ganglion neurons. Using reverse transcriptase polymerase chain reaction ( RT- PCR), in situ hybridization and immunofluorescence experiments on rat lumbar dorsal root ganglion, we provide the first demonstration that versican is produced by neurons. In addition, by probing Western blots with splice variant-specific antibodies we show that the IB4-binding versican contains only the glycosaminoglycan alpha domain. Our data support V2 as the versican isoform that renders this subpopulation of nociceptors IB4-positive (+). [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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