| Autor: |
Murata, Kenta, Yoshino, Yuta, Tsuruma, Kazuhiro, Moriguchi, Shigeki, Oyagi, Atsushi, Tanaka, Hirotaka, Ishisaka, Mitsue, Shimazawa, Masamitsu, Fukunaga, Kohji, Hara, Hideaki |
| Předmět: |
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| Zdroj: |
Journal of Neurochemistry; Mar2015, Vol. 132 Issue 5, p583-594, 12p |
| Abstrakt: |
Glycoprotein nonmelanoma protein B (GPNMB, alias osteoactivin), a type I transmembrane glycoprotein, is cleaved by extracellular proteases, resulting in release of an extracellular fragment (ECF). GPNMB is widely expressed by neurons within the CNS, including the hippocampus; however, its function in the brain remains unknown. Here, we investigated the role of GPNMB in memory and learning by using transgenic (Tg) mice over-expressing GPNMB (Tg mice on a BDF-1 background) and ECF-treated mice. In the hippocampus of both wild-type and Tg mice, GPNMB was highly expressed in neurons and astrocytes. Tg mice exhibited memory improvements in two types of learning tasks but were impaired in a passive-avoidance test. In Tg mice, the hippocampus displayed increased levels of the a-amino-3-hydroxy-5-methylisoxazole-4-propionate receptor subunit GluA1. Intracerebroventricular administration of ECF (50 ng) to Institute of Cancer Research (ICR) mice also improved memory in a passive-avoidance test and increased hippocampal GluA1 levels 24 h after treatment. In Tg mice and ECF (0.25 ig/mL)- treated hippocampal slices, long-term potentiation was promoted. These findings suggest that GPNMB may be a novel target for research on higher order brain functions. [ABSTRACT FROM AUTHOR] |
| Databáze: |
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