Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces.

Autor:	Carnevale, Silvana, Pantano, María Laura, Kamenetzky, Laura, Malandrini, Jorge Bruno, Soria, Claudia Cecilia, Velásquez, Jorge Néstor
Předmět:	FASCIOLIASIS MOLECULAR diagnosis RUMINANTS POLYMERASE chain reaction CYTOCHROME oxidase OOCYSTS
Zdroj:	Acta Parasitologica; Jun2015, Vol. 60 Issue 2, p211-217, 7p
Abstrakt:	Fasciolosis is an important parasitic zoonosis considered the most important helminth infection of ruminants in tropical countries. The aim of this study was to develop a PCR assay for the sensitive and specific detection of F. hepatica in formalin preserved sheep faeces. A 405-bp fragment of the cytochrome c oxidase subunit 1 gene of F. hepatica was amplified from stool samples of infected sheep. The PCR assay showed a detection limit of 20 pg of F. hepatica DNA. No cross-reactions were observed with samples containing coccidian oocysts or gastrointestinal nematodes eggs. Our PCR technique showed to be effective for specific detection of F. hepatica infections in sheep. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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