| Autor: |
Santerre, J., Kolitz, E., Pal, R., Rogow, J., Werner, D. |
| Předmět: |
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| Zdroj: |
Neurochemical Research; May2015, Vol. 40 Issue 5, p1023-1031, 9p |
| Abstrakt: |
Ethanol consumption typically begins during adolescence, a developmental period which exhibits many age-dependent differences in ethanol behavioral sensitivity. Protein kinase C (PKC) activity is largely implicated in ethanol-behaviors, and our previous work indicates that regulation of novel PKC isoforms likely contributes to decreased high-dose ethanol sensitivity during adolescence. The cytoplasmic Phospholipase A (cPLA) signaling cascade selectivity modulates novel and atypical PKC isoform activity, as well as adolescent ethanol hypnotic sensitivity. Therefore, the current study was designed to ascertain adolescent cPLA activity both basally and in response to ethanol, as well as it's involvement in ethanol-induced PKC isoform translocation patterns. cPLA expression was elevated during adolescence, and activity was increased only in adolescents following high-dose ethanol administration. Novel, but not atypical PKC isoforms translocate to cytosolic regions following high-dose ethanol administration. Inhibiting cPLA with AACOCF blocked ethanol-induced PKC cytosolic translocation. Finally, inhibition of novel, but not atypical, PKC isoforms when cPLA activity was elevated, modulated adolescent high-dose ethanol-sensitivity. These data suggest that the cPLA/PKC pathway contributes to the acute behavioral effects of ethanol during adolescence. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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