| Autor: |
Palazon, Francisco, Géhin, Thomas, Ferrah, Djawhar, Garnier, Anaïs, Botella, Claude, Grenet, Geneviève, Souteyrand, Éliane, Cloarec, Jean‐Pierre, Chevolot, Yann |
| Předmět: |
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| Zdroj: |
Surface & Interface Analysis: SIA; Jun2015, Vol. 47 Issue 6, p719-722, 4p |
| Abstrakt: |
Oligo ethylene glycol layers are widely used in biosensor applications, mainly for their anti-fouling abilities. Such surfaces are often characterized by X-ray photoelectron spectroscopy (XPS) as this method allows a precise determination of the surface chemical composition. We show herein that X-rays used during XPS characterization quickly and significantly degrade oligo ethylene glycol immobilized onto silica substrates. It is therefore necessary to introduce a correcting factor to assess the true (i.e. without degradation) corresponding ether contribution in the XPS spectrum of such organic layers. Eventually, fluorescence scans show the loss of anti-fouling properties of the degraded surface, leading to greater amounts of adsorbed (fluorescently labeled) protein. Copyright © 2015 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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