Genomic integrity of the Y chromosome sequence-taggedsites in infertile and Down syndrome Jordanian males.

Autor: Yasin, S. R., Tahtamouni, L. H., Najeeb, N. S., Issa, N. M., Al–Mazaydeh, Z. A., Alfaouri, A. A.
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Zdroj: Andrologia; Sep2014, Vol. 46 Issue 7, p770-776, 7p
Abstrakt: The long arm of the Y chromosome contains nonoverlapping regions termed azoospermia factor (AZF) with great influence on male fertility. Microdeletions at these regions minimise the males' ability to father offsprings. In this preliminary study, we attempted to screen the presence or absence of twenty Y chromosome's sequence-tagged sites (STS) associated with fertility in infertile and Down syndrome (DS) males. Genomic DNA from 35 fertile, 74 infertile and 22 karyotyped DS males was extracted and amplified in multiplex polymerase chain reaction (PCR) containing 20 primer pairs that amplify Y-specific STS that cover functional regions associated with AZF and spermatogenesisrelated genes. Our results indicated the integrity of the Y chromosome at the 20 fertility markers for both the fertile and Down syndrome males. However, the results of the infertile males showed the presence of microdeletions at these Y-specific STS. Three samples showed Y chromosome microdeletion when blood and seminal fluid genomic DNA were assayed, while two samples showed microdeletion only when seminal fluid genomic DNA was assayed. The current study demonstrated that the molecular genetic aspect of infertility should be given proper attention when dealing with infertility cases. Furthermore, our results indicate the importance of genetic counselling in managing infertility cases. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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