| Abstrakt: |
Objective To infer the phylogenetic relationship among Viviparidae, and to assess the phylogenetic position of M. melanioides. Methods Sequence mitochondrial COI and 16s gene of M. melanioides, Bellamya quadrata and Cipangopaludina cathayensis collected from Yunnan plateau of China were tested. Kimura-2 parameters including genetic distance and Ts/Tv ratios were generated, and the molecular phylogenetic trees of 26 species in Viviaprdae, including M. melanioides, were established by using Maximum likelihood (ML), maximum parsimony (MP) and Bayesian inference (Bl) methods. Results The 3rd codon position of COI, which get high saturation, had negative impact on phylogenetic anaysis. The nearest genetic neighbor of M. melanioides in Viviparidae was C. cathayensis among DNA sequence set with COI, or COI-1st and COI-2nd codon positons, or 16S gene.In using MP, ML and BI methods, M. melanioides and C. cathayensis form one phylogenetic clade, but Bellamya species from china and Africa were in different phylogenetic units. Conclusion C. cathayensis is closest to M. melanioides, and molecular data of COI and 16S gene could cover shortage of phylogenetic research based on morphological characters in viviparidae. [ABSTRACT FROM AUTHOR] |
