| Autor: |
von der Hardt, Katharina, Kandler, Michael Andreas, Fink, Ludger, Schoof, Ellen, Dötsch, Jörg, Bohle, Rainer Maria, Rascher, Wolfgang |
| Předmět: |
|
| Zdroj: |
American Journal of Physiology: Lung Cellular & Molecular Physiology; Jul2003, Vol. 29 Issue 1, pL55, 8p, 12 Color Photographs, 2 Charts, 8 Graphs |
| Abstrakt: |
The aim of this study was to identify cell types involved in the anti-inflammatory effect of ventilation with perfluorocarbon in vivo. Fifteen anesthetized, surfactant-depleted piglets received either aerosolized perfluorocarbon (Aerosol-PFC), partial liquid ventilation (rLV) at functional residual capacity (FRC) volume (FRCPLV), or intermittent mandatory ventilation (control). After laser-assisted microdissection of different lung cell types, mRNA expression of IL-8 and ICAM-1 was determined using TaqMan real-time PCR normalized to hypoxanthine phosphoribosyltransferase (HPRT). IL-8 mRNA expression (means ± SE; control vs. Aerosol-PFC) was 356 ± 142 copies IL-8 mRNA/copy HPRT mRNA vs. 3.5 ± 1.8 in alveolar macrophages (P <0.01); 208 ± 108 vs. 2.7 ± 0.8 in bronchiolar epithelial cells (P <0.05); 26 ± 11 vs. 0.7 ± 0.2 in alveolar septum cells (P <0.01); 2.8 ± 1.0 vs. 0.8 ± 0.4 in bronchiolar smooth muscle cells (P <0.05); and 1.1 ± 0.4 vs. 0.2 ± 0.05 in vascular smooth muscle cells (P <0.05). With FRC-PLV, IL-8/HPRT mRNA expression was significantly lower in macrophages, bronchiolar epithelial, and vascular smooth muscle cells. ICAM-1 mRNA expression in vascular endothelial cells remained unchanged. Predominantly, alveolar macrophages and bronchiolar epithelial cells were involved in the inflammatory pulmonary process. The anti-inflammatory effect of Aerosol-PFC was most pronounced. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
