Isoliquiritigenin Induces Caspase-Dependent Apoptosis via Downregulation of HPV16 E6 Expression in Cervical Cancer Ca Ski Cells.
| Autor: | Hirchaud, Fabienne, Hermetet, François, Ablise, Mourboul, Fauconnet, Sylvie, Vuitton, Dominique A., Prétet, Jean-Luc, Mougin, Christiane |
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| Předmět: |
PROTEIN analysis
ENZYME metabolism IMMUNOGLOBULIN analysis TUMOR prevention CERVIX uteri tumors CARCINOGENESIS ALTERNATIVE medicine ANTINEOPLASTIC agents APOPTOSIS BIOLOGICAL models BIOLOGICAL transport PHYSICAL & theoretical chemistry FLOW cytometry GLYCYRRHIZA IMMUNOBLOTTING MITOCHONDRIA POLYMERASE chain reaction PROBABILITY theory RESEARCH funding STAINS & staining (Microscopy) STATISTICS WOMEN'S health PLANT extracts DATA analysis REVERSE transcriptase polymerase chain reaction DATA analysis software DESCRIPTIVE statistics IN vitro studies PHARMACODYNAMICS |
| Zdroj: | Planta Medica; Nov2013, Vol. 79 Issue 17, p1628-1635, 8p |
| Abstrakt: | Flavonoids have antitumoral properties and may be attractive candidates as anticancer therapy. Isoliquiritigenin which is a constituent of licorice (Glycyrrhiza inflata), a plant commonly used in traditional Uyghur medicine in Xinjiang, China, was studied for antiproliferative and apoptotic activity in human cervical cancer cells, Ca Ski, SiHa, HeLa, and C-33A. Its molecular mechanism of action was specifically examined in Ca Ski cells. Isoliquiritigenin decreased cell viability, induced cell accumulation in G2/M and morphological and biochemical features of apoptosis in the four cancer cell lines. In Ca Ski cells, isoliquiritigenin led to a downregulation of HPV16 E6 expression associated with an increase of p53 and p21 levels, enhanced expression of Bax and decreased expression of Bcl-2 and Bid proform triggering dissipation of the mitochondrial membrane potential, released cytochrome c to the cytosol followed by activation of caspase cascadewith cleavage of caspase-9, caspase-3, and PARP. Caspase-8 was also cleaved. Moreover treatment with a pan-caspase inhibitor prevented apoptosis. As Ca Ski cells are representative of carcinoma naturally occurring in the cervix, our results suggest a potential benefit of isoliquiritigenin for cervical cancer prevention and treatment. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
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