| Autor: |
Wenk, Markus R, Lucast, Louise, Di Paolo, Gilbert, Romanelli, Anthony J, Suchy, Sharon F, Nussbaum, Robert L, Cline, Gary W, Shulman, Gerald I, McMurray, Walter, De Camilli, Pietro |
| Předmět: |
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| Zdroj: |
Nature Biotechnology; Jul2003, Vol. 21 Issue 7, p813, 5p |
| Abstrakt: |
Phosphoinositides (phosphorylated derivatives of phosphatidylinositol, PI) are versatile intracellular signaling lipids whose occurrence in low concentrations complicates direct mass measurements[SUP1-3]. Here we present a sensitive method to detect, identify and quantify phosphatidylinositol (phosphate (PIP) and phosphatidylinositol bisphosphate (PIP[SUB2]) with different fatty acid compositions (phosphoinositide profiles) in total lipid extracts by electrospray ionization mass spectrometry (ESI-MS). Using this method, we detected (elevated concentrations of PIP[SUB2] in human fibroblasts from patients with Lowe syndrome, a genetic disorder that affects phosphoinositide metabolism[SUP4]. Saccharomyces cerevisiae cells deficient in enzymes involved in PIP metabolism-Sac1p, a phosphoinositide phosphatase[SUP5], and Vps34p and Pik1p, a PI 3-kinase[SUP6] and PI 4-kinase[SUP7], respectively-showed not only different PIP concentrations but also differential changes in PIP profiles indicating metabolic and/or subcellular pooling. Mass spectrometric analysis of phosphoinositides offers unique advantages over existing approaches and may represent a powerful diagnostic tool for human diseases that involve defective phosphoinositide metabolism. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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