Down-regulation of monocarboxylate transporter 1 (MCT1) gene expression in the colon of piglets is linked to bacterial protein fermentation and pro-inflammatory cytokine-mediated signalling.
| Autor: | Villodre Tudela, Carmen, Boudry, Christelle, Stumpff, Friederike, Aschenbach, Jörg R., Vahjen, Wilfried, Zentek, Jürgen, Pieper, Robert |
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| Předmět: |
GUT microbiome
GENES ANALYSIS of variance ANIMAL experimentation BIOLOGICAL models BIOLOGICAL transport BIOPHYSICS COLON (Anatomy) COMPARATIVE studies STATISTICAL correlation CYTOKINES HISTAMINE HISTOLOGICAL techniques INTERFERONS INTERLEUKINS LACTIC acid RESEARCH methodology PROBABILITY theory DIETARY proteins RESEARCH funding STATISTICS SWINE TUMOR necrosis factors DATA analysis DATA analysis software DESCRIPTIVE statistics IN vitro studies MANN Whitney U Test |
| Zdroj: | British Journal of Nutrition; 2/28/2015, Vol. 113 Issue 4, p610-617, 8p |
| Abstrakt: | The present study investigated the influence of bacterial metabolites on monocarboxylate transporter 1 (MCT1) expression in pigs using in vivo, ex vivo and in vitro approaches. Piglets (n 24) were fed high-protein (26 %) or low-protein (18 %) diets with or without fermentable carbohydrates. Colonic digesta samples were analysed for a broad range of bacterial metabolites. The expression of MCT1, TNF-α, interferon γ (IFN-γ) and IL-8 was determined in colonic tissue. The expression of MCT1 was lower and of TNF-α and IL-8 was higher with high-protein diets (P< 0·05). MCT1 expression was positively correlated with l-lactate, whereas negatively correlated with NH3 and putrescine (P< 0·05). The expression of IL-8 and TNF-α was negatively correlated with l-lactate and positively correlated with NH3 and putrescine, whereas the expression of IFN-γ was positively correlated with histamine and 4-ethylphenol (P< 0·05). Subsequently, porcine colonic tissue and Caco-2 cells were incubated with Na-butyrate, NH4Cl or TNF-α as selected bacterial metabolites or mediators of inflammation. Colonic MCT1 expression was higher after incubation with Na-butyrate (P< 0·05) and lower after incubation with NH4Cl or TNF-α (P< 0·05). Incubation of Caco-2 cells with increasing concentrations of these metabolites confirmed the up-regulation of MCT1 expression by Na-butyrate (linear, P< 0·05) and down-regulation by TNF-α and NH4Cl (linear, P< 0·05). The high-protein diet decreased the expression of MCT1 in the colon of pigs, which appears to be linked to NH3- and TNF-α-mediated signalling. [ABSTRACT FROM PUBLISHER] |
| Databáze: | Complementary Index |
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