| Autor: |
Heiduschka, Gregor, Erovic, Boban M., Vormittag, Laurenz, Skoda, Christiane, Martinek, Helga, Brunner, Markus, Ehrenberger, Klaus, Thurnher, Dietmar |
| Zdroj: |
Archives of Otolaryngology - Head & Neck Surgery; Mar2009, Vol. 135 Issue 3, p261-267, 7p |
| Abstrakt: |
Objective: To determine whether treatment with 7β-hydroxycholesterol (7β-HC) would trigger cell death in head and neck squamous cell carcinoma (HNSCC) cell lines in a dose-dependent fashion. Design: In vitro study. Subjects: The study included HNSCC cell lines SCC9, SCC25, CAL27, and FaDu. Intervention: We treated HNSCC cell lines with increasing doses of 7β-HC. Proliferation assays were performed to assess cell viability after treatment. Western blots were carried out to evaluate cyclooxygenase (COX)-1 and -2 expression levels. Results: Using proliferation assays and immunocytochemical analysis, we detected significant growth inhibition via apoptosis in 4 different HNSCC cell lines after treatment with 7β-HC (P<.001). The 50% inhibitory concentration levels were between 13.19 and 20.79 µmol/L after 72 hours. Western analysis indicated that COX-2, but not COX-1, levels were suppressed after treatment. Conclusions: Treatment with 7β-HC resulted in suppression of HNSCC growth in vitro. Our data warrant further investigations for the potential use of 7β-HC as a cytotoxic agent in head and neck cancer. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
