| Autor: |
ZHOU Yang, LIU Wei, SUN Shi, HAN Tian - Fu, HOU Wen -sheng |
| Předmět: |
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| Zdroj: |
Chinese Journal of Oil Crop Sciences; 2014, Vol. 36 Issue 6, p707-712, 6p |
| Abstrakt: |
WUS plays an essential role in plant regeneration. To preliminarily understand the function of Gm- WUS, the related bioinformatics analysis was conducted based on the soybean database. GmWUS and AtWUS proteins were different in the acidic region, while other domains were conservative. Meanwhile, GmWUSa full - length sequence had been cloned. The protein mainly localized in the nucleus, showing that it might be a homeodomain transcription factor. The expression of GmWUS in cotyledons had been detected by qPCR during seeds germination. The results showed that both GmWUSa and GmWUSb expression levels kept rising, but GmWUSa expression decreased in the late stage. GmWUS expression in cotyledon node during cluster buds induction shown an approximate periodic pattern, indicating that GmWUS involved cluster buds diferentiation and regeneration. Comparing the expression of GmWUSa with GmWUSb, they might play a temporal complementary in their functions. The results in this study will help to investigate roles of GmWUS in soybean regeneration, to explain the mechanism of cluster buds formation, and to promote soybean regenerative capacity so as to improve the genetic transformation efficiency by overexpressing this gene. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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