| Autor: |
Taketa, Midori, Nakagawa, Hanae, Habukawa, Mao, Osuka, Hisao, Kihira, Kiyohito, Komori, Hirofumi, Shibata, Naoki, Ishii, Masaharu, Igarashi, Yasuo, Nishihara, Hirofumi, Yoon, Ki-Seok, Ogo, Seiji, Shomura, Yasuhito, Higuchi, Yoshiki |
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| Zdroj: |
Acta Crystallographica: Section F, Structural Biology Communications; Jan2015, Vol. 71 Issue 1, p96-99, 4p |
| Abstrakt: |
NAD+-reducing [NiFe] hydrogenases catalyze the oxidoreduction of dihydrogen concomitant with the interconversion of NAD+ and NADH. Here, the isolation, purification and crystallization of the NAD+-reducing [NiFe] hydrogenase from Hydrogenophilus thermoluteolus TH-1 are reported. Crystals of the NAD+-reducing [NiFe] hydrogenase were obtained within one week from a solution containing polyethylene glycol using the sitting-drop vapour-diffusion method and micro-seeding. The crystal diffracted to 2.58 Å resolution and belonged to space group C2, with unit-cell parameters a = 131.43, b = 189.71, c = 124.59 Å, β = 109.42°. Assuming the presence of two NAD+-reducing [NiFe] hydrogenase molecules in the asymmetric unit, VM was calculated to be 2.2 Å3 Da−1, which corresponds to a solvent content of 43%. Initial phases were determined by the single-wavelength anomalous dispersion method using the anomalous signal from the Fe atoms. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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