| Autor: |
Man, Yan, Peng, Guang, Lv, Xuefei, Liang, Yanli, Wang, Yun, Chen, Ye, Deng, Yulin |
| Zdroj: |
Chromatographia; Feb2015, Vol. 78 Issue 3/4, p157-162, 6p |
| Abstrakt: |
Based on the successful graft of P( N-isopropylacrylamide-4-vinylphenylboronic acid) [P(NIPAAm-co-VPBA)] on glass substrates by surface-initiated atom transfer radical polymerization, a microfludic chip with the performance of thermoresponsive boronate affinity was fabricated and was used for capture-release of the cis-diol biomolecule adenosine. The copolymers modified successfully on the surface of glass substrate were characterized by Fourier-transform infrared spectroscopy and X-ray photoelectron spectroscopy. The results demonstrated that, by simply adjusting the temperature from 4 to 55 °C, the microchip grafted with P(NIPAAm-co-VPBA) was specific to the cis-diol-containing molecules, and can selectively and quickly realize the capture and release of the cis-diol biomolecule adenosine from the mixture of adenosine and deoxyadenosine, which was validated by high performance liquid chromatography/electronic spray ion-ion trap mass spectrometry (HPLC/ESI-ion trap MS). Moreover, less than 10 min was taken from injection to the collection of the sample at the end of the channel. With further development, the P(NIPAAm-co-VPBA)-grafted microchip with high selectivity and repeatability may be chosen as a promising tool for the enrichment of the cis-diol-containing biomolecules, such as glycoproteins, carbohydrates, glycosylated peptides, nucleosides, etc. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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