| Autor: |
Morrow, J. L., Frommer, M., Shearman, D. C. A., Riegler, M. |
| Předmět: |
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| Zdroj: |
Environmental Microbiology; Dec2014, Vol. 16 Issue 12, p3622-3637, 16p |
| Abstrakt: |
W olbachia are endosymbiotic bacteria that infect 40-65% of arthropod species. They are primarily maternally inherited with occasional horizontal transmission for which limited direct ecological evidence exists. We detected W olbachia in 8 out of 24 Australian tephritid species. Here, we have used multilocus sequence typing ( MLST) to further characterize these W olbachia strains, plus a novel quantitative polymerase chain reaction method for allele assignment in multiple infections. Based on five MLST loci and the W olbachia surface protein gene ( wsp), five B actrocera and one D acus species harboured two identical strains as double infections; furthermore, B actrocera neohumeralis harboured both of these as single or double infections, and sibling species B . tryoni harboured one. Two B actrocera species contained W olbachia pseudogenes, potentially within the fruit fly genomes. A fruit fly parasitoid, F opius arisanus shared identical alleles with two W olbachia strains detected in one B . frauenfeldi individual. We report an unprecedented high incidence of four shared W olbachia strains in eight host species from two trophic levels. This suggests frequent exposure to W olbachia in this tropical tephritid community that shares host plant and parasitoid species, and also includes species that hybridize. Such insect communities may act as horizontal transmission platforms that contribute to the ubiquity of the otherwise maternally inherited W olbachia. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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