| Autor: |
Ogden, Samantha J., Horton, Jeffrey K., Stubbs, Simon L., Tatnell, Peter J. |
| Předmět: |
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| Zdroj: |
Journal of Forensic Sciences; Jan2015 Supplement, Vol. 60, pS207-S212, 6p |
| Abstrakt: |
The 1.2 mm Electric Coring Tool (e- Core™) was developed to increase the throughput of FTA™ sample collection cards used during forensic workflows and is similar to a 1.2 mm Harris manual micro-punch for sampling dried blood spots. Direct short tandem repeat ( STR) DNA profiling was used to compare samples taken by the e- Core tool with those taken by the manual micro-punch. The performance of the e- Core device was evaluated using a commercially available Power Plex™ 18 D STR System. In addition, an analysis was performed that investigated the potential carryover of DNA via the e- Core punch from one FTA disc to another. This contamination study was carried out using Applied Biosystems Ampfl STR™ Identifiler™ Direct PCR Amplification kits. The e- Core instrument does not contaminate FTA discs when a cleaning punch is used following excision of discs containing samples and generates STR profiles that are comparable to those generated by the manual micro-punch. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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