Resonance-enhanced microfluidic impedance cytometer for detection of single bacteria.

Autor: Haandbæk, Niels, With, Oliver, Bürgel, Sebastian C., Heer, Flavio, Hierlemann, Andreas
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Zdroj: Lab on a Chip; 2014, Vol. 14 Issue 17, p3313-3324, 12p
Abstrakt: This paper reports on a novel impedance-based cytometer, which can detect and characterize sub-micrometer particles and cells passing through a microfluidic channel. The cytometer incorporates a resonator, which is constructed by means of a discrete inductor in series with the measurement electrodes in the microfluidic channel. The use of a resonator increases the sensitivity of the system in comparison to state-of-the-art devices. We demonstrate the functionality and sensitivity of the cytometer by discriminating E. coli and B. subtilis from beads of similar sizes by means of the resonance-enhanced phase shift of the current through the microfluidic channel. The phase shift can be correlated to size and dielectric properties of the measured objects. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index