| Autor: |
Macente, Sara, Leite, Clarice Queico Fujimura, Santos, Adolfo Carlos Barreto, Siqueira, Vera Lucia Dias, Machado, Luzia Neri Cosmo, Marcondes, Nadir Rodrigues, Mario Hiroyuki Hirata, Hirata, Rosario Dominguez Crespo, Cardoso, Rosilene Fressatti |
| Zdroj: |
BioMed Research International; 2013, Vol. 2013, p1-6, 6p |
| Abstrakt: |
Current study evaluated the hsp65 Nested PCR Restriction Fragment Length Polymorphism Analysis (hsp65 Nested PCR-PRA) to detect and identify Mycobacterium tuberculosis complex directly in clinical samples for a rapid and specific diagnosis of tuberculosis (TB). hsp65 Nested PCR-PRA was applied directly to 218 clinical samples obtained from 127 patients suspected of TB or another mycobacterial infection from July 2009 to July 2010. The hsp65 Nested PCR-PRA showed 100% sensitivity and 95.0 and 93.1% specificity in comparison with culture and microscopy (acid fast bacillus smear), respectively. hsp65 Nested PCR-PRA was shown to be a fast and reliable assay for diagnosing TB, which may contribute towards a fast diagnosis that could help the selection of appropriate chemotherapeutic and early epidemiological management of the cases which are of paramount importance in a high TB burden country. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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