| Autor: |
Sippel JE, Mamay HK, Weiss E, Joseph SW, Beasley WJ |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of clinical microbiology [J Clin Microbiol] 1978 Apr; Vol. 7 (4), pp. 372-8. |
| DOI: |
10.1128/jcm.7.4.372-378.1978 |
| Abstrakt: |
Outer membrane protein preparations were obtained from strains of Salmonella and Neisseria meningitidis. Solubilized cell envelope (CE) fractions from S. typhi and Salmonella groups A, B, C, and E had very similar electrophoretic mobilities on polyacrylamide gel, and common antigens were demonstrated by immunodiffusion. CE appeared to be a more satisfactory antigen than the more purified preparation (T/TEI) in the enzyme-linked immunosorbent assay (ELISA) with sera from typhoid and paratyphoid patients. With either antigen, however, the presence of antibodies was demonstrated in acute- and vonvalescent-phase sera. In the case of N. meningitidis infections, the crude (STA) and the more purified antigens (T/TEI) were equally satisfactory, and a rise in antibody titer could easily be demonstrated with paired acute- and convalescent-phase sera. The ELISA appears to be a simple but highly sensitive test for the detection of antibodies by using outer membrane protein antigens. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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