| Autor: |
Myers LC; Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA., Gustafsson CM, Hayashibara KC, Brown PO, Kornberg RD |
| Jazyk: |
angličtina |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1999 Jan 05; Vol. 96 (1), pp. 67-72. |
| DOI: |
10.1073/pnas.96.1.67 |
| Abstrakt: |
Deletion of any one of three subunits of the yeast Mediator of transcriptional regulation, Med2, Pgd1 (Hrs1), and Sin4, abolished activation by Gal4-VP16 in vitro. By contrast, other Mediator functions, stimulation of basal transcription and of TFIIH kinase activity, were unaffected. A different but overlapping Mediator subunit dependence was found for activation by Gcn4. The genetic requirements for activation in vivo were closely coincident with those in vitro. A whole genome expression profile of a Deltamed2 strain showed diminished transcription of a subset of inducible genes but only minor effects on "basal" transcription. These findings make an important connection between transcriptional activation in vitro and in vivo, and identify Mediator as a "global" transcriptional coactivator. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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