Globin chain synthesis analysis by high performance liquid chromatography in the screening of thalassemia syndromes.

Autor: Galanello R; Istituto di Clinica e Biologia dell'Età Evolutiva, Università degli Studi di Cagliari Ospedale Regionale Microcitemie, Italia., Satta S, Pirroni MG, Travi M, Maccioni L
Jazyk: angličtina
Zdroj: Hemoglobin [Hemoglobin] 1998 Sep-Nov; Vol. 22 (5-6), pp. 501-8.
DOI: 10.3109/03630269809071547
Abstrakt: We applied reversed phase high performance liquid chromatography for globin chain synthesis analysis in screening for beta-thalassemia. The alpha/non-alpha-globin chain synthesis ratios have been determined in alpha-, beta-, and deltabeta-thalassemia carriers using the classical carboxymethyl cellulose chromatography as the reference method. Reversed phase high performance liquid chromatography is fast, accurate, and reproducible, and may be a suitable alternative for the traditional carboxymethyl cellulose chromatography.
Databáze: MEDLINE