| Autor: |
MacGregor RR; Infectious Diseases Division, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania. 19104-6073, USA. macgregr@mail.med.upenn.edu, Dreyer K, Herman S, Hocknell PK, Nghiem L, Tevere VJ, Williams AL |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of clinical microbiology [J Clin Microbiol] 1999 Jan; Vol. 37 (1), pp. 90-4. |
| DOI: |
10.1128/JCM.37.1.90-94.1999 |
| Abstrakt: |
We evaluated the sensitivity and specificity of a PCR-based qualitative test for the rapid diagnosis of Mycobacterium avium-M. intracellulare complex (MAC) bacteremia in patients with AIDS disease. Eleven subjects with newly culture-proven MAC bacteremia had the following tests performed at biweekly intervals during the first 8 weeks of therapy: blood culture, Mycobacterium-specific PCR, and quantitative human immunodeficiency virus (HIV) viral-load testing. Mycobacterium genus-specific biotinylated primers were used to amplify a sequence of approximately 582 nucleotides within the 16S rRNA genes of M. avium and M. intracellulare. Detection of the amplified product was performed with an oligonucleotide probe-coated microwell plate combined with an avidin-horseradish peroxidase-tetramethylbenzidine conjugate-substrate system. While not as sensitive as BACTEC culture, PCR detected 17 of 18 specimens which grew >/=40 organisms/ml (94.4% sensitivity) and 9 of 16 specimens which grew
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| Databáze: |
MEDLINE |
| Externí odkaz: |
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