| Autor: |
Loutsch JM; LSU Eye Center, Department of Ophthalmology, Microbiology and Immunology, and Department of Pharmacology, Louisiana State University Medical Center School of Medicine, New Orleans, Louisiana 70112-2234, USA., Perng GC, Hill JM, Zheng X, Marquart ME, Block TM, Ghiasi H, Nesburn AB, Wechsler SL |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of virology [J Virol] 1999 Jan; Vol. 73 (1), pp. 767-71. |
| DOI: |
10.1128/JVI.73.1.767-771.1999 |
| Abstrakt: |
The herpes simplex virus type 1 (HSV-1) LAT gene is the only viral gene abundantly transcribed during latency. LAT null mutants created with strains McKrae and 17syn+ are impaired for both in vivo spontaneous and in vivo-induced reactivation. Thus, LAT is essential for efficient in vivo-induced and spontaneous reactivation. Different investigators have studied two LAT mutants containing a StyI-StyI region deletion corresponding to LAT nucleotides 76 to 447. One mutant, dLAT371 (parent strain, McKrae), had parental high frequencies of spontaneous reactivation. In vivo-induced reactivation was not examined. The other mutant, 17DeltaSty (parent strain, 17syn+), had parental frequencies of in vitro reactivation following cocultivation of explanted ganglia but reduced frequencies of in vivo-induced reactivation. Spontaneous reactivation frequency was not reported for 17DeltaSty. These combined results suggested the possibility that in vivo spontaneous reactivation and in vivo-induced reactivation may map to different regions within the LAT domain. We now report that dLAT371 has in vivo-induced reactivation frequencies of the parent and that 17DeltaSty has reduced frequencies of in vivo spontaneous reactivation. Thus, dLAT371 demonstrated the parental phenotype for both in vivo spontaneous and -induced reactivation while the apparently identical 17DeltaSty was impaired for both in vivo spontaneous and -induced reactivation. These results suggest that one or more differences between the genetic backgrounds of McKrae and 17syn+ result in different in vivo reactivation phenotypes of otherwise identical deletion mutations and that McKrae may have compensating sequences sufficient to overcome the loss of the StyI-StyI region of the LAT transcript. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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