| Autor: |
Odell GV; Department of Biochemistry and Molecular Biology, School of Veterinary Medicine, Oklahoma State University, Stillwater 74078, USA., Ferry PC, Vick LM, Fenton AW, Decker LS, Cowell RL, Ownby CL, Gutierrez JM |
| Jazyk: |
angličtina |
| Zdroj: |
Toxicon : official journal of the International Society on Toxinology [Toxicon] 1998 Dec; Vol. 36 (12), pp. 1801-6. |
| DOI: |
10.1016/s0041-0101(98)00084-1 |
| Abstrakt: |
Thirty snake venoms had a citrate content of 2.3 to 12.9%, dry basis, by an aconitase isocitric dehydrogenase coupled enzyme assay. This is a venom concentration range of approximately 30 to 150 mM citrate assuming 25% venom solids content. Inhibition of snake venom protease activity by the addition of exogenous citrate was obtained using azure blue hide powder and azocasein as substrates. Protease inhibitions of 7.5% for Crotalus atrox venom to 78% for Bothrops picadoi venom were observed with citrate. Complete inhibition of snake venom protease activity by citrate was not observed. Bothrops asper (Pacifico) venom showed a 41% protease inhibition by citrate with azocasein as the substrate and 46% inhibition of Bothrops asper (Alantico) venom protease with azure blue hide power as a substrate. Trypsin was not inhibited in this system. Citrate may inhibit some venom protease activity by forming a complex with the zinc of zinc-dependent enzymes. reserved. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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