| Abstrakt: |
A description of instruments for investigation of spectral, polarizational and kinetic characteristics of fluorescence that have been designed and constructed, and are in operation at the Institute of Cytology is presented. The spectrofluorimeter with the steady state excitation allows recording fluorescence spectra, spectral parameter A, the value of fluorescence polarization, and fluorescence polarization excitation and emission spectra in the wavelength range of 250-500 and 300-600 nm, respectively. The pulse spectrofluorimeter is designed to record fluorescence decay curves and fluorescence anisotropy decay curves in the same spectral region. A procedure of decay curve deconvolution in multiexponential approximation within the frame of the least square method, using the Marquardt algorithm, is described in detail. The lower limit of the determined fluorescence life-time is 0.5 ns. |
