| Autor: |
Porumb H; Laboratoire de Spectroscopie Bio-moléculaire, Université Paris-Nord, Bobigny, France. porumb@infobiogen.fr, Gousset H, Taillandier E |
| Jazyk: |
angličtina |
| Zdroj: |
Electrophoresis [Electrophoresis] 1998 Oct; Vol. 19 (14), pp. 2389-90. |
| DOI: |
10.1002/elps.1150191404 |
| Abstrakt: |
Because of the repetitive nature of the sequence, when titrating a G,A-rich, triple helix-forming oligonucleotide (TFO) with increasing concentrations of target duplex in order to obtain the dissociation constant of the complex, a duplex is sometimes first generated at intermediate concentrations of the target. This duplex is based on an imperfect Watson-Crick pairing of the TFO to the pyrimidine-rich strand of the target. An explanation is proposed for this duplex being obtained only in a certain domain of the titration range, before the triple helix becomes predominant. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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