| Autor: |
Sarkisian KA, Shipulin GA, Vorob'eva MS, Shipulina OIu, Shakhgil'dian VI, Kravchenko AV, Buravtseva EV, Serebrovskaia LV, Pokrovskiĭ VV, Korystova EV |
| Jazyk: |
ruština |
| Zdroj: |
Voprosy virusologii [Vopr Virusol] 1998 Jul-Aug; Vol. 43 (4), pp. 187-90. |
| Abstrakt: |
Sensitivity and specificity of the Russian PCR test system (experimental lot) were assessed with materials from 107 Russian patients with HIV infection and 100 donors without specific antibodies to HIV-1. The electrophoretic and hybridization-enzymatic methods for amplicon detection were compared. The sensitivity of electrophoresis was 89.9%, specificity 91%; for hybridization-enzymatic detection these values were 96.1 and 100%, respectively. Samples which yielded false-negative results were repeatedly tested by two-staged amplification (nested PCR) with external and internal primers. The main cause of false-negative results was a low concentration of proviral HIV-1 DNA in some blood samples. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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