| Autor: |
Eckels KH, Brandt WE, Harrison VR, McCown JM, Russell PK |
| Jazyk: |
angličtina |
| Zdroj: |
Infection and immunity [Infect Immun] 1976 Nov; Vol. 14 (5), pp. 1221-7. |
| DOI: |
10.1128/iai.14.5.1221-1227.1976 |
| Abstrakt: |
Dengue virus, type 2, in viremic human sera and after passage in cell cultures produces mixtures of small and large plaques when assayed in LLC-MK2 cells. Clones of dengue virus type 2 obtained by plaque selection in primary green monkey kidney cell cultures were tested for temperature sensitivity in vitro and for virulence by intracerebral inoculation of suckling mice. Sublines of a small-plaque clone were found to have lower nonpermissive temperatures than the parent virus by both plaque formation and release of infectious virus into the culture media. Small-plaque sublines were significantly less virulent in suckling mice than was the parent virus. Sublines from a large-plaque clone were not temperature sensitive and closely resembled parent virus mixed-plaque morphology. When small-plaque sublines were serially passaged using undiluted inocula, reversion occurred as evidenced by the appearance of large plaques and return of mouse virulence. Small-plaque virus could be maintained through several serial passages without reversion by using low-input inocula. Desirable passage history as well as temperature-sensitive and attentuation characteristics of the S-1 small-plaque subline make it appear suitable as a vaccine candidate virus. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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