| Autor: |
Wallace EM; Department of Obstetrics and Gynaecology, Monash University, Monash Medical Centre, Clayton, Victoria, Australia. Euan.Wallace@med.monash.edu.au, Crossley JA, Ritoe SC, Aitken DA, Spencer K, Groome NP |
| Jazyk: |
angličtina |
| Zdroj: |
Annals of clinical biochemistry [Ann Clin Biochem] 1998 Sep; Vol. 35 ( Pt 5), pp. 656-64. |
| DOI: |
10.1177/000456329803500510 |
| Abstrakt: |
The development of a sensitive and specific enzyme-linked immunoassay (ELISA) for inhibin A stimulated the observation that inhibin A was a useful prenatal marker of Down's syndrome. Modifications of that ELISA, in terms of preassay sample treatment, detection methods and standard preparation, were subsequently introduced to improve assay performance and reduce costs. These modified formats have been validated and reported. We describe the modifications in detail, explaining the rationale for each, and report the results of a study directly comparing the various ELISA formats in terms of assay performance when applied to clinical samples and ability to differentiate between normal and Down's syndrome pregnancies. A format involving sample pretreatment with sodium dodecylsulphate at 100 degrees C was found to give better assay performance and a modest improvement in discrimination between Down's syndrome samples and controls, and we recommend this format for use by other investigators. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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