| Autor: |
Johnson DB; Midwest Research Institute, Kansas City, Missouri 64110, USA., Engel JF, Murrill EE, Guire PE, Barker CW, Bardwell CM, Shan AY, Swanson MJ, Stone-Heurner JG |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical biochemistry [Anal Biochem] 1978 Nov; Vol. 91 (1), pp. 138-45. |
| DOI: |
10.1016/0003-2697(78)90824-2 |
| Abstrakt: |
The aglycone, 3-hydroxybenzo[a]pyrene, was metabolized to 3-benzo[a]pyrenyl-beta-D-glucopyranosiduronic acid in the presence of uridine 5'-diphosphoglucuronic acid and rabbit liver microsomes. The course of the biosynthetic reaction was followed by fluorimetry and reverse-phase, paired-ion high pressure liquid chromatography (HPLC). Also, the HPLC system was used to analyze for glucuronide and 3-hydroxybenzo[a]pyrene during the isolation procedure. The existence of a glucuronide of 3-hydroxybenzo[a]pyrene was determined by radiotracer and enzymic techniques, utilizing the HPLC system. Field desorption and direct inlet mass spectral techniques were used to characterize the 3-hydroxybenzo[a]pyrene glucuronide. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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