| Autor: |
Bare DJ; Department of Psychiatry, Institute of Psychiatric Research, Indiana University School of Medicine, Indianapolis 46202-4887, USA., McKinzie JH, McBride WJ |
| Jazyk: |
angličtina |
| Zdroj: |
Alcoholism, clinical and experimental research [Alcohol Clin Exp Res] 1998 Sep; Vol. 22 (6), pp. 1272-6. |
| Abstrakt: |
This study was designed to examine the effects of acute intraperitoneal (i.p.) ethanol injection on the extracellular levels of serotonin (5-HT) in the ventral hippocampus (vHIP) and to determine whether a single prior exposure to ethanol could alter the response to a second dose of ethanol given 24 hr later. In the first experiment, in vivo microdialysis coupled with high pressure liquid chromatography-electrochemical detection (HPLC-EC) was used to assess the effects of 1.0, 1.75, and 2.5 g/kg ethanol on vHIP 5-HT extracellular levels in ethanol-naïve adult male Wistar rats. The largest dose significantly increased the extracellular concentration of 5-HT (p < 0.001) to a maximum of approximately 180% of baseline values within 50 min; thereafter, the levels of 5-HT began to return toward baseline. The 1.75 g/kg dose also transiently increased 5-HT levels above baseline; however, no significant increase was observed with 1.0 g/kg ethanol. The results of the second experiment demonstrated that the i.p. dose of 2.5 g/kg ethanol had no significant effect on the extracellular levels of 5-HT if rats had been given a single i.p. 2.5 g/kg dose of ethanol 24 hr earlier. Because the vHIP receives a major 5HT input from the median raphe nucleus (MRN), the results suggest that acute ethanol activates the MRN 5-HT system projecting to the vHIP and that rapid tolerance develops to the activating effects of alcohol on this pathway. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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