| Autor: |
Eisenthal A; Pathology Institute, Sourasky Medical Center, Tel-Aviv, Israel., Marder O, Maymon B, Misonzhnik F, Skornick Y, Brazowski E, Czernobilsky B, Walt H, Lifschitz-Mercer B |
| Jazyk: |
angličtina |
| Zdroj: |
Pathobiology : journal of immunopathology, molecular and cellular biology [Pathobiology] 1998; Vol. 66 (5), pp. 205-8. |
| DOI: |
10.1159/000028024 |
| Abstrakt: |
The ICAM-1 molecule plays a role in the interaction of NK cells with a variety of tumor cells, including carcinoma, melanoma and glioblastoma cells. In the present study, we analyzed the effect of IFN-gamma and TNF-alpha on both the expression of HLA-DR and ICAM-1 molecules on HGCN (Germa-2), and on their susceptibility to lysis by LAK cells. Our results show that 1,000 U/ml IFN-gamma induced a substantial increase in the expression of both ICAM-1 molecules and HLA-DR on the cell surface, while the effect of TNF-alpha on the expression of these molecules was substantially less prominent. When Germa-2 cells, previously exposed to 1,000 U/ml IFN-gamma, were employed as target cells in a 4-hour 51Cr release assay, a statistically significant increase in the lysis by LAK cells was noted. These results show that in the presence of IFN-gamma, Germa-2 tumor cells undergo modulation which affects both the expression of ICAM-1 and HLA-DR molecules as well as their susceptibility to lysis by LAK cells. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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