| Autor: |
Choi B; Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, Louisiana 70112, USA., Gatti PJ, Haislip AM, Fermin CD, Garry RF |
| Jazyk: |
angličtina |
| Zdroj: |
Virology [Virology] 1998 Aug 01; Vol. 247 (2), pp. 189-99. |
| DOI: |
10.1006/viro.1998.9251 |
| Abstrakt: |
Acute infection of CD4+ lymphoid cells by human immunodeficiency virus type 1 (HIV-1) induces an increase in the intracellular concentration of potassium (K+). Media containing reduced or elevated concentrations of K+ were used to investigate the role of this ion in HIV-1 production and cytopathology. Incubation of CD4+ lymphoblastoid cells acutely infected by HIV-1 (strain LAI) in low K+ medium resulted in an approximately 50% decrease in HIV-1 production and markedly diminished HIV-1 induced cytopathic effects (CPE) relative to cells incubated in medium containing a normal K+ concentration (approximately 5 mM). Incubation of HIV-1 infected cells in media containing elevated concentrations of K+ medium. Cells mM) increased HIV-1 production by two- to fivefold over the amount produced in cells incubated in normal K+ medium. Cells incubated in high K+ media also displayed enhanced HIV-1-induced cytopathology. The decrease in HIV-1 production by low K+ medium and increase by high K+ media could be a accounted for by effects on HIV-1 reverse transcription. However, low K+ medium inhibited HIV-1 protein synthesis and high K+ media increased HIV-1 protein synthesis. These results suggest that the HIV-1-induced increase in intracellular is required for efficient viral replication and to induce cytopathology. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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