| Autor: |
Ichibangase Y; Department of Clinical Immunology, Medical Institute of Bioregulation, Kyushu University, Beppu City, Oita, Japan., Yamamoto M, Yasuda M, Houki N, Nobunaga M |
| Jazyk: |
angličtina |
| Zdroj: |
Clinical rheumatology [Clin Rheumatol] 1998; Vol. 17 (3), pp. 214-8. |
| DOI: |
10.1007/BF01451050 |
| Abstrakt: |
The expression of metallothionein, an intracellular heavy-metal-binding protein, and p-glycoprotein, an energy-dependent drug efflux pump, was examined to study the mechanism of cell resistance to gold sodium thiomalate (GST). THP-1, one of the monocyte-derived cell lines, was cultured for 6 months and resistance to 25 microg/ml of GST (GST-resistant cells) was thus induced. The GST-resistant cells were then cultured with bucillamine to examine the presence of cross-resistance. The intracellular GST concentration was examined by flameless atomic absorption spectroscopy. The cell viability was determined by the uptake of 3-4,5 dimethylthiazole-2,5 diphenyl tetrazolium bromide (MTT). The expression of p-glycoprotein was detected by Western blotting using monoclonal anti-p-glycoprotein antibody. The expression of metallothionein was detected using the indirect immunofluorescence technique. GST-resistant cells did not show any cross-resistance to bucillamine. The rate of cytoplasmic GST accumulation decreased in the GST-resistant cells, while the rate of GST efflux also decreased. The expression of p-glycoprotein in the GST-resistant cells was not significantly different from that in the cells not treated with GST. On the other hand, the GST-resistant cells showed a higher expression of metallothionein than cells not treated with GST. These findings suggest that the induced resistance to GST might partly be due to an induction of metallothionein. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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