| Autor: |
Khoursheed M; Department of Surgery, Electron-Microscopy Unit, Faculty of Medicine, Kuwait University, Kuwait., Krajci D, Oriowo MA, Kadavil E, Philip EK, Thulesius O |
| Jazyk: |
angličtina |
| Zdroj: |
Digestion [Digestion] 1998 Jul-Aug; Vol. 59 (4), pp. 335-42. |
| DOI: |
10.1159/000007512 |
| Abstrakt: |
The purpose of this study was to investigate the ultrastructure of the local nerve supply of ovine gallbladders as well as the functional characteristics of inhibitory nerves. We used electron microscopy of thin sections of ovine gallbladders and in vitro isometric tension recording using gallbladder strips. Specifically, we measured contractile and inhibitory responses induced by transmural electrical field stimulation (EFS). We found a ganglionated plexus with intramural nerve cells and interconnecting axons. Clear and large dense-core vesicles colocalized in axons close to smooth muscle cells. EFS elicited gallbladder contractions which were converted to relaxation after atropine. EFS-induced relaxation was reduced by the nitric oxide (NO) synthase inhibitor, L-NOARG and blocked by propranolol and/or tetrodotoxin. In conclusion, enteric ganglia and neurones with synaptic vesicles (clear and dense core) were detected close to smooth muscle bundles. Neural inhibition of gallbladder contraction was mediated by beta-adrenoceptors coupled to NO generation. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
