Structural characterization of the galactoxylomannan of Cryptococcus neoformans Cap67.

6) -galactan backbone. The polysaccharide is branched at c-3 of alternate Gal units of the backbone. C-3 is the point of attachment of the oligosaccharide side chains comprised of alpha-D-Man- (1-->3)-alpha-D-Man-(1-->4)- beta-D-Gal-substituted with zero to three terminal beta-Xyl residues as shown in the following structure: [formula: see text]. -->
Grant Information: AI31769 United States AI NIAID NIH HHS
Substance Nomenclature: 0 (Polysaccharides)
0 (Polysaccharides, Bacterial)
70903-49-0 (galactoxylomannan)
Entry Date(s): Date Created: 19980806 Date Completed: 19981001 Latest Revision: 20190813
Update Code: 20221213
DOI: 10.1016/s0008-6215(97)10058-1
PMID: 9691456
Autor: Vaishnav VV; Department of Chemistry (LBCS), Georgia State University, Atlanta 30303-3083, USA., Bacon BE, O'Neill M, Cherniak R
Jazyk: angličtina
Zdroj: Carbohydrate research [Carbohydr Res] 1998 Jan; Vol. 306 (1-2), pp. 315-30.
DOI: 10.1016/s0008-6215(97)10058-1
Abstrakt: The galactoxylomannan (GalXM) obtained from the culture supernatant of an acapsular mutant of Cryptococcus neoformans Cap67 was purified by Concanavalin A affinity, ion-exchange, and gel-filtration chromatographies. The structure of GalXM was determined by methylation analysis and by 1D and 2D NMR spectroscopic studies of the intact polysaccharide and of the oligosaccharide fragments generated by Smith degradation and by acetolysis. GalXM is a complex polysaccharide with an alpha-(1-->6) -galactan backbone. The polysaccharide is branched at c-3 of alternate Gal units of the backbone. C-3 is the point of attachment of the oligosaccharide side chains comprised of alpha-D-Man- (1-->3)-alpha-D-Man-(1-->4)- beta-D-Gal-substituted with zero to three terminal beta-Xyl residues as shown in the following structure: [formula: see text].
Databáze: MEDLINE
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