| Autor: |
Willson VJ; Department of Physical and Metabolic Sciences, Astra Charnwood, Loughborough, UK., Preston JR, Lockley WJ, Singh J, Wilkinson D |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 1998 Aug; Vol. 17 (4-5), pp. 793-800. |
| DOI: |
10.1016/s0731-7085(97)00272-0 |
| Abstrakt: |
A radioimmunoassay has been developed for the determination of AR-C15849KF, a CCK-8 analogue, in human plasma. The method incorporates solid-phase sample extraction, is suitable for the determination of the analyte at pg ml-1 concentrations and is based on a method developed and validated for dog plasma. The solid-phase extraction, using ion-exchange aminopropyl and octadecyl sorbents sequentially, was retained for this procedure to remove matrix interferences in the plasma and to enhance method sensitivity. The calibration range is 10-500 pg ml-1, using a 1 ml sample of undiluted human plasma. The method has been successfully used to generate early human pharmacokinetic data during a programme of exploratory development. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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