| Autor: |
Pak Y; Department of Biochemistry, Gyeongsang National University, Chinju, Korea. ybpak@nongae.gsnu.ac.kr, Hong Y, Kim S, Piccariello T, Farese RV, Larner J |
| Jazyk: |
angličtina |
| Zdroj: |
Molecules and cells [Mol Cells] 1998 Jun 30; Vol. 8 (3), pp. 301-9. |
| Abstrakt: |
We report our comparative studies of myo- and chiro-[3H]inositol distribution in control nondiabetic Wistar and spontaneous nonobese insulin-resistant Type II diabetic G.K. rat tissues following 78-h labeling period. From various tissue extracts, free inositols, inositol phosphates, and inositol phospholipids were isolated and analyzed. Our findings demonstrate (1) a significant difference in the metabolism of myo- and chiro-inositol in normal Wistar and G.K. rats, (2) a severe defect in conversion of myo-[3H]inositol to chiro-[3H]inositol at the inositol phospholipid level of insulin-sensitive tissues of the G.K. rat, (3) an increased incorporation of myo-[3H]inositol into purified inositol phospholipids of the G.K. rat consistent with a decreased conversion to chiro-[3H]inositol, (4) the presumed presence of a pathway which incorporates chiro-[3H]inositol into inositol phospholipids, and which is overactive in the G.K. rat compared to the Wistar rat, and (5) no or minimal conversion of chiro-[3H]inositol to myo-[3H]inositol. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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