| Autor: |
Melvin SL; Virus Discovery Group, Experimental Biology Research, Abbott Diagnostic Division, Abbott Laboratories, North Chicago, IL 60064, USA., Dawson GJ, Carrick RJ, Schlauder GG, Heynen CA, Mushahwar IK |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of virological methods [J Virol Methods] 1998 Apr; Vol. 71 (2), pp. 147-57. |
| DOI: |
10.1016/s0166-0934(97)00199-7 |
| Abstrakt: |
Viral characterization studies were carried out on GB virus C (GBV-C) RNA positive plasma from normal human donors and from donors co-infected with GBV-C and hepatitis C virus (HCV). GBV-C RNA was detected by reverse-transcriptase polymerase chain reaction (RT-PCR) and probe hybridization in a single tube assay. Sequential filtration of GBV-C positive plasma indicated that GBV-C RNA is associated with a particle 50-100 nm in diameter. The peak of GBV-C RNA in sucrose gradients was observed at a buoyant density of 1.05-1.13 g/ml. GBV-C RNA titer was reduced following treatment with chloroform or with five detergents indicating that GBV-C has a lipid-containing envelope. Sucrose density gradients and self-forming cesium chloride gradients of detergent-treated GBV-C showed a shift in the RNA peak to heavier buoyant density only when RNase inhibitor (RNasin) and high detergent concentrations were present. The treated material was non-filterable and the RNA had a density of > 1.5 gm/ml. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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