| Autor: |
Fitzgerald NB; Abbott Laboratories, North Chicago, Illinois 60064, USA., English RS, Lampel JS, Vanden Boom TJ |
| Jazyk: |
angličtina |
| Zdroj: |
Applied and environmental microbiology [Appl Environ Microbiol] 1998 Apr; Vol. 64 (4), pp. 1580-3. |
| DOI: |
10.1128/AEM.64.4.1580-1583.1998 |
| Abstrakt: |
We report the development of an electrotransformation method applicable to all strains of Saccharopolyspora erythraea examined to date. Vegetatively grown mycelia were rendered electrocompetent by subjecting mycelial suspensions to ultrasound pulses. The protocol provides an alternative route for the introduction of DNA into filamentous microorganisms otherwise recalcitrant to transformation techniques. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
