| Autor: |
Budagyan VM; Immunology Institute, Ministry of Public Health, Moscow, Russia., Bulanova EG, Sharova NI, Nikonova MF, Stanislav ML, Yarylin AA |
| Jazyk: |
angličtina |
| Zdroj: |
Immunology letters [Immunol Lett] 1998 Jan; Vol. 60 (1), pp. 1-5. |
| DOI: |
10.1016/s0165-2478(97)00128-4 |
| Abstrakt: |
In this paper we show the differential sensitivity of phytohemagglutinine (PHA) activated T-cells from healthy donors or patients with systemic lupus erythematosus (SLE) to apoptosis induced by human thymic stromal cell line of epithelial origin. T-cells from SLE patients were mainly resistant to the apoptotic action of the stromal cells, while normal T-lymphocytes readily died via apoptosis. Gel electrophoresis revealed a DNA fragmentation pattern characteristic of apoptosis after 18 h of coculture. The simultaneous measurement of [3H]-thymidine uptake showed that the proliferative response of T-cells from SLE patients was significantly decreased compared to their normal counterparts. Such difference may account for the distinct result of interactions between the stromal and lymphoid cells, leading to the subsequent survival of T-lymphocytes from SLE patients. Nevertheless pretreatment of normal activated T-lymphocytes with anti-Fas mAbs, which have the capacity to substantially inhibit signaling through this receptor resulted in abolition of this form of programmed cell death. Thus, the precise role of Fas receptor and its ligand in this in vitro test system needs further investigation. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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