Physical identification of a chromosomal locus encoding biosynthetic genes for the lipopeptide calcium-dependent antibiotic (CDA) of Streptomyces coelicolor A3(2).

Autor: Chong PP; Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology (UMIST), PO Box 88, Manchester M60 1QD, UK., Podmore SM; Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology (UMIST), PO Box 88, Manchester M60 1QD, UK., Kieser HM; John Innes Centre, Norwich NR4 7UH, UK., Redenbach M; Department of Genetics, Genome Research Unit, Kaiserslautern University, 67663 Kaiserslautern, Germany., Turgay K; FB Chemie, Philipps-Universität, Hans-Meerwein-Strasse, 35032 Marburg, Germany., Marahiel M; FB Chemie, Philipps-Universität, Hans-Meerwein-Strasse, 35032 Marburg, Germany., Hopwood DA; John Innes Centre, Norwich NR4 7UH, UK., Smith CP; Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology (UMIST), PO Box 88, Manchester M60 1QD, UK.
Jazyk: angličtina
Zdroj: Microbiology (Reading, England) [Microbiology (Reading)] 1998 Jan; Vol. 144 ( Pt 1), pp. 193-199.
DOI: 10.1099/00221287-144-1-193
Abstrakt: Putative peptide-synthetase-encoding DNA fragments were isolated from the Streptomyces coelicolor A3(2) chromosome using a PCR-based approach and mapped to a single approximately 35 kb segment. In integrative transformation experiments, DNA fragments from this region disrupted production of the calcium-dependent antibiotic (CDA) and had sequences characteristic of non-ribosomal peptide synthetases, thus proving that the cda locus had been cloned.
Databáze: MEDLINE
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