Characterization of signal transduction through the TCR-zeta chain following T cell stimulation with analogue peptides of type II collagen 260-267.

N), were completely unable to induce tolerance, while analogues that caused a partial phosphorylation, such as B6 (CII 251-270s267Q-->T) and A3 (CII 245-270s269P-->A), induced partial tolerance judged by intermediate degrees of suppression of arthritis. We conclude that discrete alterations in specific amino acid residues of antigenic peptides had profound effects on T cell signaling and that the signaling correlated with T cell cytokine secretion and T cell function in the induction of tolerance and suppression of arthritis. -->
Grant Information: AR-39166 United States AR NIAMS NIH HHS; AR-43589 United States AR NIAMS NIH HHS
Substance Nomenclature: 0 (Antibodies)
0 (Membrane Proteins)
0 (Peptide Fragments)
0 (Receptors, Antigen, T-Cell)
0 (antigen T cell receptor, zeta chain)
9007-34-5 (Collagen)
EC 2.7.10.1 (Protein-Tyrosine Kinases)
EC 2.7.10.2 (ZAP-70 Protein-Tyrosine Kinase)
EC 2.7.10.2 (Zap70 protein, mouse)
Entry Date(s): Date Created: 19980408 Date Completed: 19980421 Latest Revision: 20120605
Update Code: 20221213
PMID: 9531268
Autor: Tang B; Department of Medicine, University of Tennessee, Memphis 38163, USA., Myers LK, Rosloniec EF, Whittington KB, Stuart JM, Kang AH
Jazyk: angličtina
Zdroj: Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 1998 Apr 01; Vol. 160 (7), pp. 3135-42.
Abstrakt: The immunodominant T cell determinant of type II collagen (CII) recognized by DBA/1 mice (I-Aq) is CII 260-267. The aims of this study were to determine the role of the amino acid residues within CII 245-270 in T cell signal transduction. To that end, we utilized I-Aq-restricted, CII-specific T cell hybridomas and examined tyrosine phosphorylation of TCR-zeta following stimulation with either wild-type CII 245-270 or a panel of analogue peptides. A variety of patterns occurred, ranging from increased phosphorylation of TCR-zeta to either partial or a complete abrogation of phosphorylation. Critical substitutions also completely abrogated the phosphorylation of ZAP70, a downstream molecule in TCR-zeta signaling. Evaluation of the supernatants of the T cell hybridomas for cytokine production in response to the peptides revealed a close correlation between the induction of phosphorylation of TCR-zeta and the amount of cytokine induced. Selected analogue peptides were tested as tolerogens in neonatal mice. Analogues that did not induce the phosphorylation of zeta chain, such as B3 (CII 251-270s263F-->N), were completely unable to induce tolerance, while analogues that caused a partial phosphorylation, such as B6 (CII 251-270s267Q-->T) and A3 (CII 245-270s269P-->A), induced partial tolerance judged by intermediate degrees of suppression of arthritis. We conclude that discrete alterations in specific amino acid residues of antigenic peptides had profound effects on T cell signaling and that the signaling correlated with T cell cytokine secretion and T cell function in the induction of tolerance and suppression of arthritis.
Databáze: MEDLINE
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