| Autor: |
Taneja KL; University of Massachusetts Medical Center, Worcester, USA. |
| Jazyk: |
angličtina |
| Zdroj: |
BioTechniques [Biotechniques] 1998 Mar; Vol. 24 (3), pp. 472-6. |
| DOI: |
10.2144/98243rr02 |
| Abstrakt: |
A labeled peptide nucleic acid (PNA) antisense probe was used to study the spatial distribution of triplet repeats (CTG) in human myotonic dystrophy (DM) cells by high-resolution fluorescence in situ hybridization (FISH). It was found that transcripts containing triplet repeats were present as a number of discrete foci in the DM nuclei. Greater numbers of foci were visible with the PNA probe than a comparable DNA probe. The PNA probe was also used to visualize the triplet-repeat expansion within the DM gene located on chromosome 19. Using the intensity of the expanded triplet-repeat on the chromosomes as a reference, it was estimated there were between 15-230 RNA molecules in each focus observed in DM nuclei. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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